Human IL-13 R alpha2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6142

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB6142-100
MAB6142-SP
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Key Product Details

Species Reactivity

Human

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2725C

View all IL-13 R alpha 2 Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line CHO-derived Human IL‑13 R alpha2
Met1-Leu342
Accession # NP_000631

Specificity

Detects Human IL‑13 R alpha2 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human IL-13 R alpha2 Antibody

Detection of Human IL-13 R alpha2 by Western Blot.

Western blot shows lysates of human prostate and human placenta. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human IL-13 R alpha2 Monoclonal Antibody (Catalog # MAB6142) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for IL-13 R alpha2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human IL-13 R alpha2 by Simple WesternTM.

Simple Western lane view shows lysates of human placenta, loaded at 0.2 mg/mL. A specific band was detected for IL-13 R alpha2 at approximately 59 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human IL-13 R alpha2 Monoclonal Antibody (Catalog # MAB6142) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human IL-13 R alpha2 Antibody

Application
Recommended Usage

Simple Western

0.5 µg/mL
Sample: Human placenta

Western Blot

0.5 µg/mL
Sample: Human prostate and human placenta

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-13 R alpha 2

Interleukin‑13 Receptor alpha 2 (IL‑13 R alpha2), also known as IL‑13 binding protein, and CD213a2, is a widely expressed 55 kDa cytokine receptor that plays an important role in the Th2‑polarized immune responses characteristic of a variety of pathologies, including parasitic infections and allergic asthma (1, 2). Mature human IL‑13 R alpha2 consists of a 317 amino acid (aa) extracellular domain with three fibronectin type‑III domains, a WSxWS motif, a 20 aa transmembrane segment, and a 17 aa cytoplasmic domain (3). Within the ECD, human IL‑13 R alpha2 shares 64% and 62% aa sequence identity with mouse and rat IL‑13 R alpha2, respectively. In both mouse and human, a 40 kDa‑50 kDa soluble form of IL‑13 R alpha2 can be generated by MMP‑8 mediated shedding in vitro (4).  Although this is assumed to occur in vivo in mouse, there is no evidence that shedding occurs in human (5‑7).  In mouse, alternative splicing also leads to sIL‑13 R alpha2, but again, this phenomenon apparently does not occur in human (6‑7).  Thus, the biological effects of human IL‑13 R alpha2 would appear to be mediated exclusively by membrane IL‑13 R alpha2 (7). The biological effects of IL‑13 and IL‑4 are closely related in part due to a shared receptor system. IL‑13 binds to IL‑13 R alpha1 which then forms a signaling complex with IL‑4 R alpha (8, 9). IL‑13 R alpha2 functions as a decoy receptor by binding and internalizing IL‑13 and preventing it from signaling through the IL‑13 R alpha1/IL‑4 R alpha complex (3, 10). IL‑13 R alpha2 can also block IL‑4 induced responses by inhibiting IL‑4 bound IL‑13 R alpha1/IL‑4 R alpha receptor complexes even though it does not itself bind IL‑4 (11, 12). Aside from its decoy function, IL‑13‑activated IL‑13 R alpha2 directly promotes the development of tissue fibrosis by inducing the transcription of TGF‑ beta (13). Presumably, any human soluble IL‑13 R alpha2, if it exists, will retain its ligand binding capability and attenuate responses to IL‑13 but not to IL‑4 (11, 14). The up‑regulation of transmembrane during Th2‑biased immune responses limits the extent of those responses (15‑17).

References

  1. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
  2. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
  3. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
  4. Chen, W. et al. (2008) J. Allergy Clin. Immunol. 122:625.
  5. O’Toole, M. et al. (2008) Clin. Exp. Allergy 38:594.
  6. Chen, W. et al. (2009) J. Immunol. 183:7870.
  7. Kasaian, M.T. et al. (2011) J. Immunol. 187:561.
  8. Andrews, A.-L. et al. (2006) J. Immunol. 176:7456.
  9. Zurawski, S.M. et al. (1995) J. Biol. Chem. 270:13869.
  10. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
  11. Andrews, A.-L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  12. Rahaman, S.O. et al. (2002) Cancer Res. 62:1103.
  13. Fichtner-Feigl, S. et al. (2006) Nat. Med. 12:99.
  14. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.
  15. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
  16. Morimoto, M. et al. (2009) J. Immunol. 183:1934.
  17. Zheng, T. et al. (2008) J. Immunol. 180:522.

Long Name

Interleukin 13 Receptor alpha 2

Alternate Names

CD213a2, IL-13Ra2, IL13R alpha 2, IL13RA2

Entrez Gene IDs

3598 (Human); 16165 (Mouse)

Gene Symbol

IL13RA2

UniProt

NP_000631

Additional IL-13 R alpha 2 Products

Product Documents for Human IL-13 R alpha2 Antibody

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Product Specific Notices for Human IL-13 R alpha2 Antibody

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