Human IL-17/IL-17A PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC317P
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Hamster
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 41809
Product Specifications
Immunogen
E. coli-derived recombinant human IL-17/IL-17A
Ile20-Ala155
Accession # Q16552
Ile20-Ala155
Accession # Q16552
Specificity
Detects human IL-17/IL-17A in direct ELISAs. In direct ELISAs, approximately 12% cross-reactivity with recombinant canine IL-17/IL-17A is observed and 25%-50% reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17/IL-17A, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human IL-17/IL-17A PE-conjugated Antibody
Detection of IL‑17/IL‑17A in Th17-differentiated Human CD4+PBMCs by Flow Cytometry.
Human CD4+peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 50 ng/mL PMA, 200 ng/mL Calcium Ionomycin, 200 ng/mL LPS, 20 ng/mL Recombinant Human IL-23 (Catalog # 1290-IL), and 40 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) overnight to induce Th17 development were stained with Mouse Anti-Human IL-17/IL-17A PE-conjugated Monoclonal Antibody (Catalog # IC317P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human IL-17/IL-17A PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human CD4+ peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, Recombinant Human IL‑23 (Catalog # 1290-IL), and Recombinant Human IL‑6 (Catalog # 206-IL) to induce Th17 development were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human CD4+ peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, Recombinant Human IL‑23 (Catalog # 1290-IL), and Recombinant Human IL‑6 (Catalog # 206-IL) to induce Th17 development were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Reviewed Applications
Read 1 review rated 3 using IC317P in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-17/IL-17A
Long Name
Interleukin 17
Alternate Names
CTLA-8, CTLA8, IL-17A, IL17, IL17A
Entrez Gene IDs
Gene Symbol
IL17A
UniProt
Additional IL-17/IL-17A Products
Product Documents for Human IL-17/IL-17A PE-conjugated Antibody
Product Specific Notices for Human IL-17/IL-17A PE-conjugated Antibody
For research use only
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