Human/Primate IL-17/IL-17A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB317
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Primate
Cited:
Human, Mouse
Applications
Validated:
Dual RNAscope ISH-IHC Compatible, ELISA Capture (Matched Antibody Pair), Immunohistochemistry, Immunoprecipitation, Neutralization
Cited:
ELISA Capture, ELISA Development, ELISA Development (Capture), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Luminex Development, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 41809
Product Specifications
Immunogen
E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552
Ile20-Ala155
Accession # Q16552
Specificity
Detects human and primate IL-17 in direct ELISAs. In direct ELISAs, approximately 12% cross-reactivity with recombinant canine IL-17 is observed and 25%-50% reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human/Primate IL-17/IL-17A Antibody
IL‑17/IL‑17A in Human Crohn's Disease Intestine.
IL-17/IL-17A was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Mouse Anti-Human/Primate IL-17/IL-17A Monoclonal Antibody (Catalog # MAB317) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Immunoprecipitation of Human IL‑17.
Human IL-17 was immunoprecipitated from 100 µg of human primary differentiated Th17 cell lysate following incubation with 3 µg Mouse Anti-Human/Primate IL-17 Monoclonal Antibody (Catalog # MAB317) or isotype control antibody (Catalog # MAB004) overnight at 4 °C. IL-17-antibody complexes were absorbed using anti-mouse agarose beads. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.
IL‑6 Secretion Induced by IL‑17 and Neutralization by Human IL‑17 Antibody.
Recombinant Human IL-17 (Catalog # 317-ILB) stimulates IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Human IL-17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL-17 Monoclonal Antibody (Catalog # MAB317). The ND50 is typically 1-3 µg/mL.Applications for Human/Primate IL-17/IL-17A Antibody
Application
Recommended Usage
Dual RNAscope ISH-IHC Compatible
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human crohn's intestine
Sample: Immersion fixed paraffin-embedded sections of human crohn's intestine
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Crohn's disease intestine
Sample: Immersion fixed paraffin-embedded sections of human Crohn's disease intestine
Immunoprecipitation
3 µg/100 µg cell lysate
Sample: Human primary differentiated Th17 cells
Sample: Human primary differentiated Th17 cells
Neutralization
Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 15 ng/mL Recombinant Human IL‑17.
Human/Primate IL-17 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 5 using MAB317 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17/IL-17A
Long Name
Interleukin 17
Alternate Names
CTLA-8, CTLA8, IL-17A, IL17, IL17A
Entrez Gene IDs
Gene Symbol
IL17A
UniProt
Additional IL-17/IL-17A Products
Product Documents for Human/Primate IL-17/IL-17A Antibody
Product Specific Notices for Human/Primate IL-17/IL-17A Antibody
For research use only
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