Human IL-1 beta/IL-1F2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB601R
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
ELISA Capture (Matched Antibody Pair), Immunocytochemistry, Neutralization, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 2805R
Product Specifications
Immunogen
E. coli-derived recombinant human IL-1 beta/IL-1F2
Specificity
Detects human IL-1 beta/IL-1F2 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 4% cross-reactivity with recombinant rat (rr) IL‑1 beta and less than 0.1% with recombinant porcine (rp) IL-1 beta, recombinant human IL-1 alpha, rpIL-1 alpha, rrIL-1 alpha, recombinant mouse (rm) IL-1 alpha, and rmIL-1 beta is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-1 beta/IL-1F2 Antibody
Detection of Human IL‑1 beta/IL‑1F2 by Western Blot.
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS for 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB601R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-1 beta/IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IL‑1 beta/IL‑1F2 in Human PBMCs.
IL-1 beta/IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with 1 µg/mL LPS and 3 µM monensin for 24 hours using Mouse Anti-Human IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB601R) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human IL‑1 beta/IL‑1F2 by Simple WesternTM.
Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 200 nM and 10 μg/mL LPS for 3 hours, loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta/IL‑1F2 at approximately 37 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑1 beta/IL‑1F2 Monoclonal Antibody (Catalog # MAB601R) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human IL-1 beta/IL-1F2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with 1 μg/mL LPS and 3 μM monensin for 24 hours
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with 1 μg/mL LPS and 3 μM monensin for 24 hours
Simple Western
10 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line treated with PMA and LPS
Sample: THP‑1 human acute monocytic leukemia cell line treated with PMA and LPS
Western Blot
1 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line treated with PMA and LPS
Sample: THP‑1 human acute monocytic leukemia cell line treated with PMA and LPS
Neutralization
Measured by its ability to neutralize IL‑1 beta/IL‑1F2-induced proliferation
in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 50-200 ng/mL in the presence of 50 pg/mL Recombinant Human IL‑1 beta/IL‑1F2.
Human IL-1 beta/IL-1F2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5
mg/mL in sterile PBS.
For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
Long Name
Interleukin 1 beta
Alternate Names
IL-1b, IL-1F2, IL1 beta, IL1B
Entrez Gene IDs
Gene Symbol
IL1B
Additional IL-1 beta/IL-1F2 Products
Product Documents for Human IL-1 beta/IL-1F2 Antibody
Product Specific Notices for Human IL-1 beta/IL-1F2 Antibody
For research use only
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