Human IL-1 beta/IL-1F2 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC201P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 8516
Product Specifications
Immunogen
E. coli-derived recombinant human IL-1 beta/IL-1F2
aa 117-269
Accession # P01584
aa 117-269
Accession # P01584
Specificity
Detects human IL-1 beta/IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL‑1 beta and recombinant porcine IL‑1 beta and no cross-reactivity with recombinant rat (rr) IL‑1 beta, rmIL‑1 alpha, recombinant human IL‑1ra, rmIL‑1ra, or rrIL‑1 alpha.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IL-1 beta/IL-1F2 PE-conjugated Antibody
Detection of IL‑1 beta/IL‑1F2 in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes stimulated with LPS were stained with Mouse Anti-Human CD14 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human IL-1 beta/IL-1F2 PE-conjugated Monoclonal Antibody (Catalog # IC201P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human IL-1 beta/IL-1F2 PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood monocytes stimulated with LPS were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human peripheral blood monocytes stimulated with LPS were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-1 beta/IL-1F2
Long Name
Interleukin 1 beta
Alternate Names
IL-1b, IL-1F2, IL1 beta, IL1B
Entrez Gene IDs
Gene Symbol
IL1B
UniProt
Additional IL-1 beta/IL-1F2 Products
Product Documents for Human IL-1 beta/IL-1F2 PE-conjugated Antibody
Product Specific Notices for Human IL-1 beta/IL-1F2 PE-conjugated Antibody
For research use only
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