Human IL-1 beta/IL-1F2 (Research Grade Canakinumab Biosimilar) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10349
Recombinant Monoclonal Antibody For Research Use Only
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu110
Product Specifications
Immunogen
Human IL‑1 beta/IL‑1F2
Specificity
Detects Human IL‑1 beta/IL‑1F2 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Canakinumab. This product is for research use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images
Detection of IL-1 beta / IL-1F2 in Human blood monocytes by Flow Cytometry.
Human peripheral blood monocytes, either (A) treated with 500 ng/mL LPS overnight, or (B) untreated, were stained with Human anti-Human IL-1 beta/IL-1F2 (Research Grade Canakinumab Biosimilar) Antibody (Catalog # MAB10349) followed by APC-conjugated anti-Human IgG secondary antibody (Catalog # F0135) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). To facilitate intracellular staining, cells were fixed with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Human blood monocytes treated with LPS fixed with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012)
Sample: Human blood monocytes treated with LPS fixed with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
Long Name
Interleukin 1 beta
Alternate Names
IL-1b, IL-1F2, IL1 beta, IL1B
Entrez Gene IDs
Gene Symbol
IL1B
Additional IL-1 beta/IL-1F2 Products
Product Specific Notices
For research use only
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