Human IL-3R alpha/CD123 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF841
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑3 R alpha/CD123
Specificity
Detects human IL‑3 R alpha/CD123 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 2% cross‑reactivity with recombinant human (rh) IL-2 sR beta, rhIL-2 sR gamma, and rhIL-6 sR is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IL-3R alpha/CD123 Antibody
Detection of Human IL-3R alpha/CD123 by Western Blot.
Western blot shows lysates of HDLM-2 human Hodgkin’s lymphoma cell line, TF-1 human erythroleukemic cell line, and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-3R alpha/CD123 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF841) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-3R alpha/CD123 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human IL-3R alpha/CD123 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HDLM‑2 human Hodgkin's lymphoma cell line, TF‑1 human erythroleukemic cell line, and THP‑1 human acute monocytic leukemia cell line
Sample: HDLM‑2 human Hodgkin's lymphoma cell line, TF‑1 human erythroleukemic cell line, and THP‑1 human acute monocytic leukemia cell line
Reviewed Applications
Read 3 reviews rated 4 using AF841 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-3R alpha
Interleukin 3 is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity human IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit does not bind IL-3 by itself but is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF. Both the alpha and the beta subunits are members of the cytokine receptor superfamily.
References
- Ogorochi, T. and A. Miyajima (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, ed. Oxford University, New York p. 40.
Long Name
Interleukin 3 Receptor alpha
Alternate Names
CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA
Gene Symbol
IL3RA
Additional IL-3R alpha Products
Product Documents for Human IL-3R alpha/CD123 Antibody
Product Specific Notices for Human IL-3R alpha/CD123 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...