Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM of PMA for 24 hours followed by 10 μg/mL of LPS for 3 hours. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human IL-4I1 Monoclonal Antibody (Catalog # MAB56843) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-4I1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of IL-4l1 in THP-1 Human Cell Line by Flow Cytometry.

THP-1 human cell line was stained with Mouse Anti-Human IL-4l1 Monoclonal Antibody (Catalog # MAB56843, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human IL-4I1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: THP-1 human monocytic leukemia cell line fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012)

Western Blot

2 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line treated with 200 nM of PMA for 24 hours followed by 10 μg/mL of LPS for 3 hours

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4I1

Interleukin 4 induced protein 1 (IL-4I1), also known as protein FIG-1 and L-amino acid oxidase, is encoded by a B-cell IL-4-inducible gene, FIG1, and is highly expressed in primary metastinal B-cell lymphomas (1-4). It belongs to the flavin monoamine oxidase family, FIG1 subfamily. Enzymological characterization reveals that IL-4I1 has L-amino acid oxidase activity with preference toward aromatic amino acids. Studies have shown that hIL-4I1 inhibited the proliferation of CD3‑stimulated T lymphocytes with a similar effect on CD4(+) and CD8(+) T cells (5). Its inhibitory effect was dependent on enzymatic activity and H₂O₂ production. Its restricted expression to lymphoid tissues indicates that it may play an important function in the immune system (1, 4).

References

Chu, C.C. and W.E. Paul. (1997) Proc. Natl. Acad. Sci. USA 94:2507.
Mason, J.M. et al. (2004) J. Immunol. 173:4561.
Chavan, S.S. et al. (2002) Biochim. Biophys. Acta. 1576:70.
Copie-Bergman, C. et al. (2003) Blood 101:2756.
Boulland, M.L. et al. (2007) Blood 110:220.

Long Name

Interleukin 4-induced Protein-1

Alternate Names

FIG1, IL4I1

Entrez Gene IDs

259307 (Human); 14204 (Mouse); 308575 (Rat); 102115096 (Cynomolgus Monkey)

Gene Symbol

IL4I1

UniProt

Q96RQ9

Additional IL-4I1 Products

Product Documents for Human IL-4I1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-4I1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human IL-4I1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB56843

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all IL-4I1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IL-4I1 Antibody

Detection of Human IL‑4I1 by Western Blot.