Human Integrin alpha1/CD49a Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5676
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 639508
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived human Integrin alpha1/CD49a
Phe29-Pro1141
Accession # P56199
Phe29-Pro1141
Accession # P56199
Specificity
Detects human Integrin alpha1/CD49a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with rhIntegrin alpha2, 3, 4, 5, 6, 8, 9, 10, 11, L, M, V, X, alpha6x1/ beta4, alphaD/ beta2, alpha7x2/ beta1, alphaE/ beta7, or alpha2b/ beta3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Integrin alpha1/CD49a Antibody
Detection of Human Integrin alpha1/CD49a by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human Integrin a1/CD49a Monoclonal Antibody (Catalog # MAB5676) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin a1/CD49a at approximately 190 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Integrin alpha 1/CD49a by Western Blot
Levels of HAV RNA at 24 hpi in H1-HeLa cells engineered by CRISPR/Cas9 to be knocked-out for the indicated integrins inoculated with naked HAV (mean ± SD).Immunoblots of whole-cell lysates harvested from the indicated cell lines. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30801249), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Integrin alpha1/CD49a Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Reviewed Applications
Read 2 reviews rated 4.5 using MAB5676 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 1/CD49a
Integrin alpha1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190-210 kDa member of the integrin alpha chain family of molecules. It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium. Integrin alpha1 forms a noncovalent heterodimer with Integrin beta1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI. It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus. Mature human Integrin alpha1 is a 1151 amino acid (aa) type I transmembrane glycoprotein that contains a 1113 aa extracellular domain (ECD) and a 15 aa cytoplasmic tail. The ECD contains one vWFA/I-domain (aa 147-360) that binds collagen, plus multiple divalent cation binding sites. Potential splice variants exist that show a two and four aa substitution for aa 765-1179. Over aa 29-1141 (the ECD), human Integrin alpha1 shares 88% aa identity with mouse Integrin alpha1.
Alternate Names
CD49a, ITGA1, VLA-1
Gene Symbol
ITGA1
UniProt
Additional Integrin alpha 1/CD49a Products
Product Documents for Human Integrin alpha1/CD49a Antibody
Product Specific Notices for Human Integrin alpha1/CD49a Antibody
For research use only
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