Integrin aV/CD51 was detected in immersion fixed rat mesenchymal stem cells using Mouse Anti-Human Integrin aV/ CD51 Monoclonal Antibody (Catalog # MAB12191) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Integrin alphaV/CD51 in Huh-7 human hepatoma cell line by Flow Cytometry

Huh-7 human hepatoma cell line were stained with Mouse Anti-Human Integrin alphaV/CD51 Monoclonal Antibody (Catalog # MAB12191, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin alphaV/CD51 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Huh-7 human hepatoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed DU145 human prostate carcinoma cell line, and immersion fixed rat mesenchymal stem cells

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB12191 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)
Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha V/CD51

Integrin alphaV (CD51; also vitronectin receptor subunit alpha) is a 140‑150 kDa member of the integrin alpha-chain family of adhesion molecules. It forms disulfide-linked integral membrane heterodimers with at least five beta-chains, including beta1, 3, 5, 6 and 8. Human alphaV is a 1018 aa type I transmembrane glycoprotein that contains a 962 aa extracellular domain (ECD) and a short 32 aa cytoplasmic tail. The ECD contains seven FG (PheAlaGly)-GAP (GlyAlaPro) repeats that form a beta-propellor domain (aa 46‑483). Furin cleavage of the alphaV ECD occurs after Gly889, generating a disulfide-linked heteromeric subunit alphaV chain. alphaV-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2 and TSP. The ECD of human alphaV shares 92% and 90% aa sequence identity with mouse and rat alphaV ECD, respectively.

Alternate Names

CD51, ITGAV, MSK8, Vitronectin Receptor alpha, VNRA

Entrez Gene IDs

3685 (Human); 16410 (Mouse); 296456 (Rat); 102116249 (Cynomolgus Monkey)

Gene Symbol

ITGAV

UniProt

NP_002201

Additional Integrin alpha V/CD51 Products

Product Documents for Human Integrin alphaV/CD51 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Integrin alphaV/CD51 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry