Human IRF1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4830
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 686703
Product Summary for Human IRF1 Antibody
Immunogen
E. coli-derived recombinant human IRF1
Thr147-Pro325
Accession # P10914
Thr147-Pro325
Accession # P10914
Specificity
Detects human IRF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IRF2 (aa 151-349), rhIRF3 (aa 206-427), rhIRF4 (aa 130-451), rhIRF5 (aa 214-395), rhIRF6 (aa 176-366), rhIRF7 (aa 289-503), rhIRF8 (aa 255‑426), rhIRF9 (aa 238-393), or recombinant mouse IRF1 (aa 147-329) is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human IRF1 Antibody
Detection of Human IRF1 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Human IRF1 Monoclonal Antibody (Catalog # MAB4830) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRF1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human IRF1 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, and Jurkat human acute T cell leukemia cell line
Sample: Raji human Burkitt's lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, and Jurkat human acute T cell leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IRF1
Long Name
Interferon Regulatory Factor 1
Alternate Names
MAR
Gene Symbol
IRF1
UniProt
Additional IRF1 Products
Product Documents for Human IRF1 Antibody
Product Specific Notices for Human IRF1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...