Human ISG15/UCRP Antibody

Detection of Human ISG15/UCRP by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human ISG15/UCRP Monoclonal Antibody (Catalog # MAB4845) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ISG15/UCRP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human ISG15/UCRP by Simple Western^TM.

Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and HT-29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ISG15/UCRP at approximately 20 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human ISG15/UCRP Monoclonal Antibody (Catalog # MAB4845) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of ISG15/UCRP in Human Endometrial Cancer.

ISG15/UCRP was detected in immersion fixed paraffin-embedded sections of Human Endometrial Cancer using Mouse Anti-Human ISG15/UCRP Monoclonal Antibody (Catalog # MAB4845) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.