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Human JunB Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4456

R&D Systems, part of Bio-Techne
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MAB4456
MAB4456-SP

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 512313

Product Specifications

Immunogen

E. coli-drived recombinant human JunB
Asp145-Val246 (predicted)
Accession # P17275

Specificity

Detects human JunB in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) JunD or rhc-Jun is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human JunB Antibody

Detection of Human JunB antibody by Western Blot.

Detection of Human JunB by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human JunB Monoclonal Antibody (Catalog # MAB4456) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for JunB at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human JunB Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JunB

JunB is a member of the bzip Jun family of transcription factors. JunB binds as a homo or heterodimer, with other members of the Jun/Fos family, to the DNA element TGA[CG]TCA. Knock out mice indicate that JunB is involved in erythroid differentiation and hematopoietic stem cell viability.

Long Name

JunB Proto-oncogene

Alternate Names

activator protein 1, AP-1, jun B proto-oncogene, transcription factor jun-B

Entrez Gene IDs

3726 (Human); 16477 (Mouse); 24517 (Rat)

Gene Symbol

JUNB

UniProt

Additional JunB Products

Product Documents for Human JunB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human JunB Antibody

For research use only

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