Human Kallikrein 3/PSA Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB13441
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Neutralization
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 181827
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 3/PSA
Pro19-Pro261
Accession # NP_001639
Pro19-Pro261
Accession # NP_001639
Specificity
Detects human Kallikrein 3/PSA in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Factor X, rhHGF-A, rhKLK-4, -5, -8, -11, or rhThrombin is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Kallikrein 3/PSA Antibody
Kallikrein 3/PSA in LNCaP and MCF-7 Human Cell Lines.
Kallikrein 3/PSA was detected in immersion fixed LNCaP human prostate cancer cell line (positive control, left panel) and MCF-7 human breast cancer cell line (negative control, right panel) using Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13441) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in LNCaP cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Kallikrein 3/PSA in Human Prostate.
Kallikrein 3/PSA was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13441) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Kallikrein 3/PSA Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed LNCaP human prostate cancer cell line
Sample: Immersion fixed LNCaP human prostate cancer cell line
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate
Sample: Immersion fixed paraffin-embedded sections of human prostate
Neutralization
Measured by its ability to neutralize Recombinant Human Kallikrein 3/PSA (10 µg/mL, Catalog # 1344-SE) cleavage of the fluorogenic peptide substrate Suc-RPY-pNA (1 mM). The Neutralization Dose (ND50) is typically 21 µg/mL.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 3/PSA
Kallikrein 3 is a member of the Kallikrein family of serine proteases. It is also known as PSA (prostate-specific antigen) and is used in prostate cancer diagnosis.
Additional Kallikrein 3/PSA Products
Product Documents for Human Kallikrein 3/PSA Antibody
Product Specific Notices for Human Kallikrein 3/PSA Antibody
For research use only
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