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Human KLRG1 Alexa Fluor® 700-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB70293N

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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FAB70293N-100UG

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2388C

Product Specifications

Immunogen

 Chinese hamster ovary cell line CHO-derived recombinant  human KLRG1
Leu60-Phe195
Accession # Q96E93

Specificity

Detects human KLRG1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Human KLRG1 Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Human peripheral blood
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KLRG1

KLRG1 (killer cell lectin-like receptor G1), also called MAFA (mast cell function associated), is a 30-38 kDa type II transmembrane inhibitory glycoprotein of the C‑type lectin family, designated CLEC15A. KLRG1 cDNA encodes 195 amino acids (aa) including an intracellular ITIM motif and a 136 aa extracellular domain (ECD) with a single C‑type lectin domain. The human KLRG1 ECD shares 57% and 54% aa identity with mouse and rat KLRG1, respectively. A 189 aa isoform diverges at aa 186. KLRG1 binds E-, N- and R-cadherins and functions as an MHC‑independent means of identifying non-self pathogens and epithelial tumor cells with low E‑cadherin expression. It is expressed as a monomer or disulfide‑linked homodimer on NK and T cell subsets such as tumor-infiltrating lymphocytes.

Long Name

Killer Cell Lectin-like Receptor Subfamily G Member 1

Alternate Names

CLEC15A, MAFA, MAFAL

Entrez Gene IDs

10219 (Human); 50928 (Mouse); 58975 (Rat)

Gene Symbol

KLRG1

UniProt

Additional KLRG1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human KLRG1 Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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