Human peripheral blood was stained with (A) Rabbit anti-Human KLRG1 Monoclonal Antibody (Catalog # MAB70293) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111) and CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.

KLRG1 in Human Tonsil.

KLRG1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human KLRG1 Monoclonal Antibody (Catalog # MAB70293) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human KLRG1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB70293 in the following applications:

Immunohistochemistry-Frozen (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLRG1

KLRG1 (killer cell lectin-like receptor G1), also called MAFA (mast cell function associated), is a 30-38 kDa type II transmembrane inhibitory glycoprotein of the C‑type lectin family, designated CLEC15A. KLRG1 cDNA encodes 195 amino acids (aa) including an intracellular ITIM motif and a 136 aa extracellular domain (ECD) with a single C‑type lectin domain. The human KLRG1 ECD shares 57% and 54% aa identity with mouse and rat KLRG1, respectively. A 189 aa isoform diverges at aa 186. KLRG1 binds E-, N- and R-cadherins and functions as an MHC‑independent means of identifying non-self pathogens and epithelial tumor cells with low E‑cadherin expression. It is expressed as a monomer or disulfide‑linked homodimer on NK and T cell subsets such as tumor-infiltrating lymphocytes.

Long Name

Killer Cell Lectin-like Receptor Subfamily G Member 1

Alternate Names

CLEC15A, MAFA, MAFAL

Entrez Gene IDs

10219 (Human); 50928 (Mouse); 58975 (Rat)

Gene Symbol

KLRG1

UniProt

Q96E93

Additional KLRG1 Products

Product Documents for Human KLRG1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human KLRG1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human KLRG1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB70293

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all KLRG1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human KLRG1 Antibody

Detection of KLRG1 in Human Peripheral Blood by Flow Cytometry.