Human LAP (TGF-beta 1) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2463
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry
Cited:
Flow Cytometry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 27232
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
Leu30-Arg278 (Cys33Ser)
Accession # P01137
Leu30-Arg278 (Cys33Ser)
Accession # P01137
Specificity
Detects human LAP (TGF-beta 1) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) TGF‑ beta1, rhTGF‑ beta2, rhTGF‑ beta1.2, rhTGF‑ beta3, or rhTGF‑ alpha.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human LAP (TGF-beta 1) Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human platelets
Sample: Human platelets
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB2463 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LAP (TGF-beta 1)
The TGF‑ beta family includes several related proteins (70‑80% sequence homology) from mammalian, avian, or Xenopus systems that are now designated TGF‑ beta1, TGF‑ beta2, TGF‑ beta1.2, TGF‑ beta3, TGF‑ beta4, and TGF‑ beta5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF‑ beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF‑ beta family members are naturally associated with their own distinct LAPs.
Long Name
Latency-associated Peptide
Alternate Names
LAP (TGFbeta 1)
Entrez Gene IDs
7040 (Human)
Gene Symbol
TGFB1
UniProt
Additional LAP (TGF-beta 1) Products
Product Documents for Human LAP (TGF-beta 1) Antibody
Product Specific Notices for Human LAP (TGF-beta 1) Antibody
For research use only
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