Human LAR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3004
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 384727
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAR
Ala27-Glu1251
Accession # NP_569707
Ala27-Glu1251
Accession # NP_569707
Specificity
Detects endogenous human LAR in Western blots. In Western blots, this antibody detects both full-length protein and the cleaved LAR extracellular domain (ECD). It does not cross-react with recombinant human PTPRM, PTPRK, DEP1, or PTPR gamma.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Human LAR Antibody
Detection of Human LAR by Western Blot.
Western blot shows lysates of CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human LAR Monoclonal Antibody (Catalog # MAB3004) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for LAR at approximately 139 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human LAR Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: CHP-100 human neuroblastoma cell line
Sample: CHP-100 human neuroblastoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LAR
Leukocyte Antigen-Related (LAR) tyrosine phosphatase, also known as Protein Tyrosine Phosphatase, Receptor-type F (PTPRF), is an integral membrane protein with anon-glycosylated molecular weight of 207 kDa. The extracellular domain is cleaved near the cell membrane by a subtilisin-like endoprotease to a molecular weight of 139 kDa. Depending on cellular conditions, the extracellular domain may remain associated with the rest of the molecule or can be shed into the extracellular medium.
Long Name
Leukocyte Antigen-Related Tyrosine Phosphatase
Alternate Names
PTPRF
Gene Symbol
PTPRF
UniProt
Additional LAR Products
Product Documents for Human LAR Antibody
Product Specific Notices for Human LAR Antibody
For research use only
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