Human LEKTI/SPINK5 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF8515

R&D Systems, part of Bio-Techne
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AF8515
AF8515-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all LEKTI/SPINK5 Antibodies »

Product Specifications

Immunogen

Human embyronic kidney cell line HEK293-derived recombinant human SPINK5
Glu626-Glu1064
Accession # Q9NQ38

Specificity

Detects human SPINK5 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human LEKTI/SPINK5 Antibody

Detection of Human SPINK5 antibody by Western Blot.

Detection of Human SPINK5 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human SPINK5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8515) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SPINK5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human LEKTI/SPINK5 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LEKTI/SPINK5

LEKTI (Lympho-epithelial Kazal-type-related inhibitor; also SPINK5, Serine Protease Inhibitor Kazal-type 5) is a 150 kDa precursor protein that is cleaved into the following 2 chains: Hemofiltrate peptide HF6478 and Hemofiltrate peptide HF7665. LEKTI is a multidomain serine protease inhibitor that contains 15 Kazal-like inhibitory domains. It may play a role in skin and hair morphogenesis and anti-inflammatory and/or antimicrobial protection of mucous epithelia. Mutations may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. It contains fifteen Kazal-like domains. Mature human SPINK5 shares 60% aa sequence identity with mouse SPINK5.

Long Name

Serine Peptidase Inhibitor, Kazal Type 5

Alternate Names

LETKI, NETS, SPINK5, VAKTI

Entrez Gene IDs

11005 (Human); 72432 (Mouse); 361319 (Rat)

Gene Symbol

SPINK5

UniProt

Q9NQ38

Additional LEKTI/SPINK5 Products

Product Documents for Human LEKTI/SPINK5 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LEKTI/SPINK5 Antibody

For research use only

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