Skip to main content

Human LILRA3/CD85e Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC2574G

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
IC2574G

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ILT6/CD85e
Arg18-Glu439
Accession # Q8N6C8

Specificity

Detects human ILT6/CD85e in direct ELISAs. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) ILT2, rhILT4, ILT5 is observed, and less than 5% cross-reactivity with rhLIR5 and rhLIR7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human LILRA3/CD85e Alexa Fluor® 488-conjugated Antibody

Detection of LILRA3/CD85e antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of LILRA3/CD85e in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Goat Anti-Human LILRA3/CD85e Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2574G) or (B) Normal Goat IgG Alexa Fluor 488 Control (Catalog # IC108G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human LILRA3/CD85e Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

5 µL/106 cells
Sample: Human peripheral blood monocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: LILRA3/CD85e

Human LIR-4 (Leukocyte Ig-like receptor #4; also ILT6 and LILRA3) is an Ig superfamily member that belongs to the leukocyte receptor complex/cluster. Mature LIR-4 is 416 amino acids in length and contains four C2-type Ig-like domains. Unlike other LIR family members, LIR-4 is actively secreted. At least one alternate splice form is known which shows a 64 amino acid deletion in the C-terminal half of the second Ig-like domain. Multiple alleles exist in human, and 5-10% of the population have a defective gene which produces no LIR-4 product. Rodent LIR-4 has not been reported.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM and ITIM Domains), Member 3

Alternate Names

CD85e, HM31, HM43, ILT6, LIR4

Entrez Gene IDs

11026 (Human)

Gene Symbol

LILRA3

UniProt

Additional LILRA3/CD85e Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human LILRA3/CD85e Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Loading...
Loading...
Loading...
Loading...
Loading...