Human LILRA3/CD85e Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2574
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LILRA3/CD85e
Arg18-Glu439
Accession # Q8N6C8
Arg18-Glu439
Accession # Q8N6C8
Specificity
Detects human LILRA3/CD85e in direct ELISAs. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) ILT2, rhILT4, ILT5 is observed, and less than 5% cross-reactivity with rhLIR5 and rhLIR7 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human LILRA3/CD85e Antibody
Detection of LILRA3/CD85e in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monoctyes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Goat Anti-Human LILRA3/CD85e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2574) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human LILRA3/CD85e Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood monoctyes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human peripheral blood monoctyes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LILRA3/CD85e
Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM and ITIM Domains), Member 3
Alternate Names
CD85e, HM31, HM43, ILT6, LIR4
Entrez Gene IDs
11026 (Human)
Gene Symbol
LILRA3
UniProt
Additional LILRA3/CD85e Products
Product Documents for Human LILRA3/CD85e Antibody
Product Specific Notices for Human LILRA3/CD85e Antibody
For research use only
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