Detects human LILRB4/CD85k/ILT3 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with with recombinant human (rh) ILT5 is observed. No cross-reactivity with rhILT1, rhILT2, rhILT4, rhILT7, rhILT11, rhLIR-6 or rhLIR-8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human LILRB4/CD85k/ILT3 Antibody

Detection of LILRB4/CD85k/ILT3 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human IL-3 Ra/CD123 PE-conjugated Monoclonal Antibody (Catalog # FAB301P) and either (A) Mouse Anti-Human LILRB4/CD85k/ILT3 Monoclonal Antibody (Catalog # MAB24251) or (B) Mouse IgG_2AIsotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human LILRB4/CD85k/ILT3 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Western Blot

1 µg/mL
Sample: Recombinant Human LILRB4/CD85k/ILT3 under non-reducing conditions only

Reviewed Applications

Read 2 reviews rated 4.5 using MAB24251 in the following applications:

Western Blot (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRB4/CD85k/ILT3

Immunoglobulin-Like Transcript 3 (ILT3), also known as Leukocyte Immunoglobulin-Like Receptor subfamily B member 4 (LILRB4) and Leukocyte Immunoglobulin-like Receptor 5 (LIR-5), is a type I membrane protein that functions as a receptor for class I MHC antigens. It contains cytoplasmic ITIM motifs and is involved in the down-regulation of immune responses.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4

Alternate Names

CD85k, HM18, ILT3, LIR5

Entrez Gene IDs

11006 (Human); 14728 (Mouse); 107128256 (Cynomolgus Monkey)

Gene Symbol

LILRB4

UniProt

ABM86208

Additional LILRB4/CD85k/ILT3 Products

Product Documents for Human LILRB4/CD85k/ILT3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human LILRB4/CD85k/ILT3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human LILRB4/CD85k/ILT3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB24251

Key Product Details

Species Reactivity

Validated:

Cited:

Applications