Human LIN-41 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5104
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human LIN-41
Val386-Phe868
Accession # Q2Q1W2
Val386-Phe868
Accession # Q2Q1W2
Specificity
Detects human LIN-41 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human LIN-41 Antibody
Detection of Human LIN-41 by Western Blot.
Western blot shows lysates of BG0IV human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LIN-41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LIN-41 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
LIN‑41 in BG01V Human Stem Cells.
LIN-41 was detected in immersion fixed BG01V human embryonic stem cells using Human LIN-41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human LIN‑41 by Simple WesternTM.
Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for LIN-41 at approximately 97 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human LIN-41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human LIN-41 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Simple Western
10 µg/mL
Sample: BG01V human embryonic stem cells
Sample: BG01V human embryonic stem cells
Western Blot
1 µg/mL
Sample: BG0IV human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line
Sample: BG0IV human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LIN-41
LIN-41 (lineage-41; also TRIM/tripartite motif-containing protein 71) is a 94 kDa (predicted) cytosolic member of the RBCC (RING finger, B-Box Coiled-Coil) family of proteins. It is expressed in developing limbs, DRG and branchial arches and apparently regulates the timed expression of proliferation and differentiation-associated genes. Human LIN-41 is 868 amino acids (aa) in length. It contains a RING-type zinc finger region (aa 12-95), a His-rich segment (aa 146‑157), two B Box zinc fingers that bind DNA (aa 194-314), a coiled-coil motif that binds protein (aa 391-427), one filamin repeat and six NHL repeats that regulate gene translation (aa 593‑868). Over aa 386-868, human LIN-41 is 97%, 95%, and 94% aa identical to canine, mouse, and chicken LIN-41, respectively.
Long Name
RNA-binding Protein LIN-41
Alternate Names
LIN41, TRIM71
Gene Symbol
TRIM71
UniProt
Additional LIN-41 Products
Product Documents for Human LIN-41 Antibody
Product Specific Notices for Human LIN-41 Antibody
For research use only
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