Human LRIG2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1941
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 990310
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human LRIG-2
Gly41-Thr805
Accession # O94898
Gly41-Thr805
Accession # O94898
Specificity
Detects human LRIG-2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human LRIG2 Antibody
Detection of LRIG-2 in HEK293 Human Cell Line Transfected with Human LRIG-2 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) human LRIG-2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LRIG-2 Monoclonal Antibody (Catalog # MAB1941) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
LRIG2 in WM‑115 Human Cell Line.
LRIG2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB1941) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
LRIG2 in IMCD3 Mouse Cell Line.
LRIG2 was detected in immersion fixed IMCD3 mouse inner medullary collecting duct cell line using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB1941) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human LRIG2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human LRIG-2 and eGFP
Sample: HEK293 Human Cell Line Transfected with Human LRIG-2 and eGFP
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed WM-115 human malignant melanoma cell line and IMCD3 mouse inner medullary collecting duct cell line
Sample: Immersion fixed WM-115 human malignant melanoma cell line and IMCD3 mouse inner medullary collecting duct cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRIG2
Long Name
Leucine-rich Repeats and Immunoglobulin-like Domains 2
Alternate Names
KIAA0806, LIG2, UFS2
Gene Symbol
LRIG2
UniProt
Additional LRIG2 Products
Product Documents for Human LRIG2 Antibody
Product Specific Notices for Human LRIG2 Antibody
For research use only
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