HEK293 human embryonic kidney cell line transfected with (A) human LRIG-2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LRIG-2 Monoclonal Antibody (Catalog # MAB1941) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

LRIG2 in WM‑115 Human Cell Line.

LRIG2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB1941) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

LRIG2 in IMCD3 Mouse Cell Line.

LRIG2 was detected in immersion fixed IMCD3 mouse inner medullary collecting duct cell line using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB1941) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human LRIG2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human LRIG-2 and eGFP

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed WM-115 human malignant melanoma cell line and IMCD3 mouse inner medullary collecting duct cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRIG2

LRIG2 (leucine-rich repeats and Ig-like domains-2; also LIG-2) is an approximately 119-132 kDa glycoprotein that belongs to the LRIG gene family. The LRIG2 protein was predicted to have the same domain organization as LRIG1with a signal peptide, an extracellular part containing 15 leucine-rich repeats and three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tail. Over aa 41‑805, human LRIG2 shares 94%, 93% and 92% aa sequence identity with mouse, rat and macaque LRIG2 respectively.

Long Name

Leucine-rich Repeats and Immunoglobulin-like Domains 2

Alternate Names

KIAA0806, LIG2, UFS2

Entrez Gene IDs

9860 (Human); 269473 (Mouse); 310753 (Rat)

Gene Symbol

LRIG2

UniProt

O94898

Additional LRIG2 Products

Product Documents for Human LRIG2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human LRIG2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human LRIG2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1941

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all LRIG2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human LRIG2 Antibody

Detection of LRIG-2 in HEK293 Human Cell Line Transfected with Human LRIG-2 and eGFP by Flow Cytometry.