Human LRRC15 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11324
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2944A
Product Specifications
Immunogen
Chinese Hamster Ovary cell line, CHO-derived human LRRC15
Met1-Gly538
Accession # Q8TF66
Met1-Gly538
Accession # Q8TF66
Specificity
Detects human LRRC15 in direct ELISA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human LRRC15 Antibody
Detection of LRRC15 in HEK293 cells by Flow Cytometry.
HEK293 cells transfected with Human LRRC-15 and eGFP were stained with eGFP and either (A) Rabbit Anti-Human LRRC15 Monoclonal Antibody (Catalog # MAB11324) or (B) Normal Rabbit IgG Control (Catalog # MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.Detection of Human LRRC15 by Simple WesternTM.
Simple Western lane view shows lysates of U-87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for LRRC15 at approximately 104 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human LRRC15 Monoclonal Antibody (Catalog # MAB11324). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Detection of Human LRRC15 by Western Blot.
Western blot shows lysates of Human skin and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human LRRC15 Monoclonal Antibody (Catalog # MAB11324) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LRRC15 at approximately ~70-80kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human LRRC15 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 cells transfected with Human LRRC-15 and eGFP
Sample: HEK293 cells transfected with Human LRRC-15 and eGFP
Simple Western
20 µg/mL
Sample: Lysates of U-87 MG human glioblastoma/astrocytoma cell line
Sample: Lysates of U-87 MG human glioblastoma/astrocytoma cell line
Western Blot
2 µg/mL
Sample: Lysates of Human skin and U‑118‑MG human glioblastoma/astrocytoma cell line
Sample: Lysates of Human skin and U‑118‑MG human glioblastoma/astrocytoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRRC15
References
- Cancer Res. 2022 March 15; 82(6): 1038. doi:10.1158/0008-5472.CAN-21-0622.
- Biosci Rep. 2020 Feb 28; 40(2): BSR20193286. doi: 10.1042/BSR20193286.
Long Name
Leucine-rich Repeat Containing 15
Alternate Names
LIB
Gene Symbol
LRRC15
UniProt
Additional LRRC15 Products
Product Documents for Human LRRC15 Antibody
Product Specific Notices for Human LRRC15 Antibody
For research use only
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