HEK293 human embryonic kidney cell line transfected with (A) human LRRC52 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human LRRC52 Monoclonal Antibody (Catalog # MAB10260) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human LRRC52 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human LRRC52 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRC52

LRRC52 (Leucine-rich repeat-containing protein 52) belongs to an extracellular leucine-rich-repeat–only (Elron) cluster, which includes five other LRRC proteins: LRRC26, LRRC38, LRRC55, LRTM1 and LRTM2 (1). Elron cluster members are type I transmembrane proteins with an extracellular domain (ECD) containing several LRR motifs and a cytoplasmic C-terminal tail with a short stretch of acidic residues (1). The mature ECD of LRRC52 is 221 amino acids (aa), containing 5 LRR motifs, and shares 76% and 78% identity with mouse and rat LRRC52, respectively. LRRC-52 is dominantly expressed in testis and skeletal muscle and is also detected in several other tissues including placenta, kidney, lung, and some glands (2, 3). LRRC52 has been identified as a testes specific SLO3 K⁺ channel accessory protein in mouse (2). LRRC52 is proposed to be essential for alkalization dependent activation of the KSper current at physiological membrane potentials and pH (2). Activation of the SLO3 channel is critical in the fertilization process and loss of function results in infertility (2, 4, 5). A second Elron cluster protein, LRRC26, was recently identified as a regulatory subunit of SLO1 BK-type K⁺ channels, suggesting a general function for the entire family (6). Based on function, LRRC52 might be a therapeutic target for male contraception (7).

References

Dolan, J. et al. (2007) BMC Genomics 8:320.
Yang, C. et al. (2011) PNAS 108:19419.
Leonetti, M. et al. (2012) PNAS. 109:19274.
Navarro, B. et al. (2007) PNAS 104:7688.
Sanit, C. et al. (2010) FEBS Lett. 584:1041.
Yan, J. and Aldrich, RW. (2010) Nature 466:513.
Zeng, XH. et al. (2015) PNAS 112:2599.

Long Name

Leucine-rich Repeat Containing 52

Alternate Names

BK Channel Auxiliary Gamma Subunit LRRC52

Entrez Gene IDs

440699 (Human); 240899 (Mouse); 289199 (Rat)

Gene Symbol

LRRC52

UniProt

Q8N7C0

Additional LRRC52 Products

Product Documents for Human LRRC52 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human LRRC52 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human LRRC52 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10260

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all LRRC52 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human LRRC52 Antibody

Detection of LRRC52 in HEK293 Human Cell Line Transfected with Human LRRC52 and eGFP by Flow Cytometry