Human LYAR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6748
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 735838
Product Specifications
Immunogen
E. coli-derived recombinant human LYAR
Lys288-Lys379
Accession # Q9NX58
Lys288-Lys379
Accession # Q9NX58
Specificity
Detects human LYAR in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human LYAR Antibody
Detection of Human LYAR by Western Blot.
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, HT-29 human colon adenocarcinoma cell line, HEK293 human embryonic kidney cell line, and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LYAR Monoclonal Antibody (Catalog # MAB6748) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LYAR at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LYAR in BG01V Human Embryonic Stem Cells.
LYAR was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human LYAR Monoclonal Antibody (Catalog # MAB6748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nucleoli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human LYAR Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Western Blot
1 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line, HT‑29 human colon adenocarcinoma cell line, HEK293 human embryonic kidney cell line, and BG01V human embryonic stem cells
Sample: Ramos human Burkitt's lymphoma cell line, HT‑29 human colon adenocarcinoma cell line, HEK293 human embryonic kidney cell line, and BG01V human embryonic stem cells
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LYAR
LYAR (Ly-1/CD5 antibody reactive clone) is a 45-50 kDa nucleolar protein that was named for the ability of its antibody to cross-react with Ly-1. Its function is unclear; it is known to associate with MYCN and RRP1B, the latter association giving rise to the suggestion that LYAR is involved with RNA metabolism. Human LYAR is 379 amino acids (aa) in length. It contains two C2H2-type Zn finger regions (aa 6-25 and 33-51) followed by one coiled-coil region (aa 175-219) and an NLS (aa 217-222). There are multiple Zn-binding sites and three utilized phosphorylation sites at Ser244, Ser258 and Ser276. Over aa 288-379, human LYAR shares 75% aa identity with mouse LYAR.
Long Name
Ly1 Antibody Reactive Homolog
Alternate Names
PNAS-5
Gene Symbol
LYAR
UniProt
Additional LYAR Products
Product Documents for Human LYAR Antibody
Product Specific Notices for Human LYAR Antibody
For research use only
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