Human Lysyl Oxidase Homolog 2/LOXL2 Antibody

Detection of Human Lysyl Oxidase Homolog 2/LOXL2 by Western Blot.

Western blot shows lysates of HEC-1-B human endometrial adenocarcinoma cell line and U-87 MG human glioblastoma/astrocytoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Lysyl Oxidase Homolog 2/LOXL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2639) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lysyl Oxidase Homolog 2/LOXL2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human Lysyl Oxidase Homolog 2/LOXL2 by Simple Western^TM.

Simple Western lane view shows lysates of U-87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lysyl Oxidase Homolog 2/LOXL2 at approximately 109 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Lysyl Oxidase Homolog 2/LOXL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2639) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human Lysyl Oxidase Homolog 2/LOXL2 by Knockdown Validated

LOXL2 is responsible for increased collagen crosslinking activity in hypoxic EC‐derived exosomes. (A) LOXL2 mRNA expression is decreased in shLOXL2‐transfected EC (shLOXL2) compared to control‐infected EC (shCtrl) (n = 7 ± SD, Student's t‐test). (B) LOXL2 protein expression is decreased in shLOXL2‐transfected EC‐derived exosomes. (C) Analysis of lysyl oxidase activity in exosomes from control (shCtrl) and LOXL2 knock‐down (shLOXL2) (n = 3 ± SD, Student's t‐test). (D) Collagen gels comparing control buffer (Vehicle), and exosomes from control (Exosomes shCtrl) and LOXL2 knock‐down (Exosomes shLOXL2) EC, and (E) quantification of gel contraction after 36 hrs for these conditions (n = 3 ± SD, anova). Collagen crosslinking activity of exosomes from control and LOXL2 knock‐down EC at 20% (Exosomes Control and shLOXL2 Control) and 2% O2 (shLOXL2 Hypoxia) was assessed using (F) the in vitro lysyl oxidase assay and (G and H) the collagen gel contraction assay (n = 3 ± SD, anova). *P < 0.05; ***P < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26612622), licensed under a CC-BY license. Not internally tested by R&D Systems.