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Human MALT1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4850

R&D Systems, part of Bio-Techne
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MAB4850
MAB4850-SP

Key Product Details

Species Reactivity

Validated:

Human

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MALT1-C

Product Summary for Human MALT1 Antibody

Immunogen

Recombinant human MALT1
Asp701-Thr808
Accession # Q9UDY8

Specificity

Detects human MALT1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MALT1 Antibody

Detection of Human MALT1 antibody by Western Blot.

Detection of Human MALT1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MALT1 Monoclonal Antibody (Catalog # MAB4850) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MALT1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human MALT1 antibody by Simple WesternTM.

Detection of Human MALT1 by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MALT1 at approximately 98 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human MALT1 Monoclonal Antibody (Catalog # MAB4850). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human MALT1 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and Daudi human Burkitt's lymphoma cell line

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and Daudi human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB4850 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MALT1

Mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) is an approximately 91 kDa intracellular adaptor protein that forms a complex with Bcl10 and CARMA-1. The caspase-like domain of MALT1 cleaves Bcl10 and the NF kappaB inhibitor A20. This results in NF kappaB activation following signal transduction from cell surface ITAM-containing receptors including the T cell and B cell antigen receptors. Human MALT1 shares 87% aa sequence identity with mouse and rat MALT1.

Long Name

Mucosa Associated Lymphoid Tissue Lymphoma Translocation Gene 1

Alternate Names

MLT1, Paracaspase

Entrez Gene IDs

10892 (Human); 240354 (Mouse); 307366 (Rat)

Gene Symbol

MALT1

UniProt

Additional MALT1 Products

Product Documents for Human MALT1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human MALT1 Antibody

For research use only

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