Human MESP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9219
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2030B
Product Specifications
Immunogen
E. coli-dervived recombinant human MESP1
Met1-Gln85
Accession # Q9BRJ9
Met1-Gln85
Accession # Q9BRJ9
Specificity
Detects human MESP1 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human MESP1 Antibody
Detection of Human MESP1 by Western Blot.
Western blot shows lysates of Mouse ES cells transfected with human MESP1 untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MESP1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. Cells provided courtesy of Michael Kyba's lab, University of Minnesota.
Detection of Human MESP1 by Western Blot.
Western blot shows lysates of H1 human embryonic stem cells untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MESP1 at approximately 30 kDa (as indicated). Image provided courtesy of Michael Kyba's lab, University of Minnesota.
Human MESP1 in H1 Human Embryonic Stem Cells.
MESP1 was detected in fixed H1 human embryonic stem cells untreated (lower panel) or treated (upper panel) with Doxycycline using Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219). Cells were stained using anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Image provided courtesy of Michael Kyba's lab, University of Minnesota.Applications for Human MESP1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Fixed H1 human embryonic stem cells treated with Doxycycline
Sample: Fixed H1 human embryonic stem cells treated with Doxycycline
Western Blot
2 µg/mL
Sample: Mouse ES cells transfected with human MESP1 treated with Doxycycline and H1 human embryonic stem cells treated with Doxycycline
Sample: Mouse ES cells transfected with human MESP1 treated with Doxycycline and H1 human embryonic stem cells treated with Doxycycline
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MESP1
Mesoderm posterior protein 1 (MESP1) is a 268 amino acids protein that in humans is encoded by the MESP1 gene. MESP1 was first identified in transcripts enriched in the posterior region of the mouse embryo at embryonic day E7 to E7.5. Lineage tracing in mice showed that MESP1 represents the earliest marker of cardiac progenitors and directs multipotential cardiovascular cell fates, patterning mesoderm into cardiac, hematopoietic, or skeletal myogenic progenitors in a context-dependent manner.
Long Name
Mesoderm Posterior BHLH Transcription Factor 1
Alternate Names
bHLHc5
Gene Symbol
MESP1
UniProt
Additional MESP1 Products
Product Documents for Human MESP1 Antibody
Product Specific Notices for Human MESP1 Antibody
For research use only
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