Human mGluR5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4514
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human mGluR5
Ser19-Ser509
Accession # P41594
Ser19-Ser509
Accession # P41594
Specificity
Detects human mGluR5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human (rh) mGluR1 is observed and less than 5% cross-reactivity with rhmGluR2 and rhmGluR3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human mGluR5 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate putamen)
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate putamen)
Western Blot
0.1 µg/mL
Sample: Recombinant Human mGluR5
Sample: Recombinant Human mGluR5
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: mGluR5
Human metabotropic glutamate receptor 5 (mGluR5; also mGluR5b) is a 150 kDa, 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. mGluR5 is constitutively expressed and regulates neuronal ion channel activity. Human mGluR5 is 1212 amino acids (aa) in length and contains an extracellular domain (ECD) of 558 amino acids. Through its ECD, mGluR5 either homodimerizes or heterodimerizes with the Ca++-sensor receptor. There is one alternate splice form (mGluR5a) that shows a 32 aa deletion between aa 877‑908 in the cytoplasmic tail. Over aa 19‑509, human mGluR5 is 98% aa identical to mouse, rat and canine mGluR5.
Long Name
Metabotropic Glutamate Receptor 5
Alternate Names
GPRC1E, GRM5, mGlu5
Gene Symbol
GRM5
UniProt
Additional mGluR5 Products
Product Documents for Human mGluR5 Antibody
Product Specific Notices for Human mGluR5 Antibody
For research use only
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