K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICB Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB1599N, filled histogram) or isotype control antibody (Catalog # IC0041N, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Human MICB by Flow Cytometry

Histograms showing sample flow cytometry profiles for the ligands studied. Bars in each plot indicate the population expressing each aNKR, Gates were set using Unstained, single stained controls, fluorescence minus one, and secondary antibody alone controls. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1999-4915/9/10/295), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MICB Alexa Fluor® 700-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

5 µL/10⁶ cells
Sample: K562 human chronic myelogenous leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied in a saline solution containing BSA and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MICB

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma delta T cells, and CD8⁺ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (1-7).

References

Groh, V. et al. (2001) Nature Immunol. 2:255.
Stephens, H. (2001) Trends Immunol. 22:378.
Bauer, S. et al. (1999) Science 285:727.
Groh, V. et al. (2002) Nature 419:734.
Steinle, A. et al. (2001) Immunogenetics 53:279.
Pende, D. et al. (2002) Cancer Res. 62:6178.
Salih, H. et al. (2003) Blood 102:1389.

Long Name

MHC Class I-related Protein B

Alternate Names

MHC class I chain-related protein B, MHC class I mic-B antigen, MHC class I polypeptide-related sequence B, MIC-B, PERB11.2MHC class I-like molecule PERB11.2-IMX, stress inducible class I homolog

Entrez Gene IDs

4277 (Human)

Gene Symbol

MICB

UniProt

CAI18747

Additional MICB Products

Product Documents for Human MICB Alexa Fluor® 700-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MICB Alexa Fluor® 700-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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Flow Cytometry

Human MICB Alexa Fluor® 700-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1599N

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all MICB Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human MICB Alexa Fluor® 700-conjugated Antibody

Detection of MICB in K562 Human Cell Line by Flow Cytometry.