Human peripheral blood granulocytes were stained with Rabbit Anti-Human MICL/CLEC12A Monoclonal Antibody (Catalog # MAB29461, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

MICL/CLEC12A in U937 Human Cell Line.

MICL/CLEC12A was detected in immersion fixed U937 human histiocytic lymphoma cell line (left panel; positive staining) and Daudi human Burkitt's lymphoma cell line (right panel; negative staining) using Rabbit Anti-Human MICL/CLEC12A Monoclonal Antibody (Catalog # MAB29461) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

MICL/CLEC12A in Human Spleen.

MICL/CLEC12A was detected in immersion fixed paraffin-embedded sections of human spleen using Rabbit Anti-Human MICL/CLEC12A Monoclonal Antibody (Catalog # MAB29461) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human MICL/CLEC12A Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood granulocytes

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen

Reviewed Applications

Read 1 review rated 5 using MAB29461 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICL/CLEC12A

Human MICL (myeloid inhibitory C-type lectin-like receptor; also CLL-1 and KLRL-1) is a 75 kDa member of the group V C-type lectin-like receptor family of molecules. Group V molecules have a lectin-type domain that binds non-sugar ligands. MICL is a 265 amino acid (aa) type II transmembrane (TM) glycoprotein that contains a 200 aa extracellular domain (ECD). Multiple isoforms of MICL are reported. One potentially utilizes an alternate start site that adds 10 aa to the cytoplasmic domain. There is one potential soluble form that lacks the TM segment, and two truncated ECD isoforms. Human MICL ECD is 53%, 65%, and 55% aa identical to the ECD in mouse, dog and cow, respectively.

Long Name

Myeloid Inhibitory C-type Lectin-like Receptor

Alternate Names

CD371, CLEC12A, CLL-1, DCAL-2

Entrez Gene IDs

160364 (Human); 232413 (Mouse)

Gene Symbol

CLEC12A

UniProt

Q5QGZ9

Additional MICL/CLEC12A Products

Product Documents for Human MICL/CLEC12A Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human MICL/CLEC12A Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human MICL/CLEC12A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB29461

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all MICL/CLEC12A Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human MICL/CLEC12A Antibody

Detection of MICL/CLEC12A in Human Peripheral Blood Granulocytes by Flow Cytometry.