Human MIG2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6984

R&D Systems, part of Bio-Techne
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MAB6984
MAB6984-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 696906

View all MIG2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MIG2
Gln321-Leu444
Accession # Q96AC1

Specificity

Detects human MIG2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) MIG, rhFERMT1 (aa 514‑677), rhMIG2/FERMT2 (aa 514‑680), or rhFERMT3 (498‑667) is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human MIG2 Antibody

Detection of Human MIG2 antibody by Western Blot.

Detection of Human MIG2 by Western Blot.

Western blot shows lysates of HT1080 human fibrosarcoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human MIG2 Monoclonal Antibody (Catalog # MAB6984) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MIG2 at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human MIG2 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HT1080 human fibrosarcoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIG2

MIG2 (mitogen-inducible gene-2), also known as FERMT2 or Kindlin-2, is an  approximately 80 kDa, 680 amino acid (aa), ubiquitously expressed member of the Fermitin/Kindlin family of cytoplasmic adaptor proteins. MIG2 is essential for beta1 and beta3 integrin activity due to its interactions with regulatory molecules such as talin and ILK. MIG2 contains two FERM domains (aa 285-326 and aa 524-573) and one pleckstrin homology domain (aa 373-478). Within the region used as an immunogen, human MIG2 shares 98% aa identity with mouse and rat MIG2. Two isoforms (687 aa and 633 aa) have a 7 aa insert after aa 534. The shorter isoform also has a 2 aa substitution for the C-terminal 57 aa of the 680 aa form.

Long Name

Mitogen Inducible Gene 2

Alternate Names

FERMT2, KIND2, Kindlin-2, PLEKHC1

Entrez Gene IDs

10979 (Human); 218952 (Mouse); 289992 (Rat)

Gene Symbol

FERMT2

UniProt

Q96AC1

Additional MIG2 Products

Product Documents for Human MIG2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MIG2 Antibody

For research use only

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