Human Mimecan Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2660
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Mimecan
Ala20-Phe298
Accession # P20774
Ala20-Phe298
Accession # P20774
Specificity
Detects human Mimecan in direct ELISAs and Western blots. In these formats, approximately 5% cross‑reactivity with recombinant mouse Mimecan is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Mimecan Antibody
Detection of Human Mimecan by Western Blot.
Western blot shows lysates of human aorta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mimecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2660) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Mimecan at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Mimecan by Simple WesternTM.
Simple Western lane view shows lysates of human heart (aorta) tissue, loaded at 0.2 mg/mL. A specific band was detected for Mimecan at approximately 44 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Mimecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2660) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Mimecan Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: human heart (aorta) tissue
Sample: human heart (aorta) tissue
Western Blot
1 µg/mL
Sample: Human aorta tissue
Sample: Human aorta tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mimecan
Alternate Names
KSPG25, OGN, OIF, SLRR3A
Gene Symbol
OGN
UniProt
Additional Mimecan Products
Product Documents for Human Mimecan Antibody
Product Specific Notices for Human Mimecan Antibody
For research use only
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