Human MKK7 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3579
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MKK7
Met1-Arg419
Accession # O14733
Met1-Arg419
Accession # O14733
Specificity
Detects human MKK7 in Western blots. Does not cross-react with other recombinant human mitogen-activated protein kinase kinases.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MKK7 Antibody
Detection of Human MKK7 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, SW480 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human MKK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3579) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MKK7 was detected at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human MKK7 by Western Blot.
Western blot shows recombinant human MEKs and recombinant human MKKs Catalog#s XXX (XXX ng/lane). PVDF membrane was probed with 2 µg/mL Goat Anti-Human MKK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3579) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MKK7 was detected at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human MKK7 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil and placenta
Sample: Immersion fixed paraffin-embedded sections of human tonsil and placenta
Western Blot
2 µg/mL
Sample: A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, SW480 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and MDA-MB-468 human breast cancer cell line
Sample: A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, SW480 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and MDA-MB-468 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MKK7
Mitogen-activated protein kinase kinase 7 (MKK7 or MAP2K7), also known as MEK7 and Jun kinase kinase 2 (JNKK2), is activated by proinflammatory cytokines and environmental stresses. Activation occurs through phosphorylation at Ser271 and Thr275 by several upstream MAPK kinase kinases (MAP3Ks). MKK7 is a dual specificity protein kinase, phosphorylating and activating the JNK family of MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Pro-Tyr.
Long Name
Mitogen-activated Protein Kinase Kinase 7
Alternate Names
JNKK2, MAP2K7, MKE7, PRKMK7
Gene Symbol
MAP2K7
UniProt
Additional MKK7 Products
Product Documents for Human MKK7 Antibody
Product Specific Notices for Human MKK7 Antibody
For research use only
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