Detects the pro and active forms of human MMP-10 in direct ELISAs Western blots. In direct ELISAs and Western blots, approximately 10‑50% cross‑reactivity with recombinant human (rh) MMP-3 and no cross-reactivity with rhMMP-1, -2, -7, -8, -9, -12, or -13 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Human MMP-10 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑10 (Catalog # 910‑MP), see our available Western blot detection antibodies

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: MG-63 human osteosarcoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human MMP-10 Western Blot Standard (Catalog # WBC026)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-10

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-10 (stromelysin 2) degrades a broad range of substrates including gelatin, collagen types III, IV and V, fibronectin, aggrecan, and pig cartilage proteoglycan. MMP-10 can activate other MMPs such as MMP-1 and MMP-8. MMP-10 is expressed in keratinocytes, T cells, menstrual endometrium and a few tumor samples. Structurally, MMP-10 may be divided into four distinct domains: a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site; a short linker region, and a carboxyl terminal hemopexin-like domain.

Long Name

Matrix Metalloproteinase 10

Alternate Names

MMP10, Stromelysin 2

Entrez Gene IDs

4319 (Human)

Gene Symbol

MMP10

UniProt

P09238

Additional MMP-10 Products

Product Documents for Human MMP-10 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MMP-10 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human MMP-10 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9102

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all MMP-10 Antibodies »

Product Specifications

Immunogen

Specificity