Human MMP-10 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9101
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Neutralization, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 117239
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-10
Tyr18-Cys476
Accession # P09238
Tyr18-Cys476
Accession # P09238
Specificity
Detects the pro and active forms of human MMP-10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -12, or -13 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human MMP-10 Antibody
Detection of Human MMP-10 by Immunohistochemistry
Co-localisation of MMPs with markers of classical activation in human atherosclerotic plaques.(A) Serial sections were stained by peroxidase with anti-CD68 (A. CD68) and by dual immuno-fluorescence with anti-MMP-1 or anti-MMP-10 (red) together with anti-COX-2 or p65RelA (green) as shown, and the images were superimposed digitally. Nuclear localised p65RelA (NF-kB*) was detected because of the shift in nuclear counterstain colour from dark blue (DAPI alone) to sky blue (blue plus green) in the superimposed image. (B) Areas rich in macrophages identified from the peroxidase stain were identified in the serial section. Cells in the whole field were counted and a percentage of each staining pattern calculated. Values are means ± SEM, n = 6, * p<0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22880008), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human MMP-10 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human intestine
Sample: Immersion fixed paraffin-embedded sections of human intestine
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑10 (Catalog # 910‑MP), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑10 (Catalog # 910‑MP), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human MMP-10 Western Blot Standard (Catalog # WBC026)
Sample: Recombinant Human MMP-10 Western Blot Standard (Catalog # WBC026)
Reviewed Applications
Read 1 review rated 1 using MAB9101 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-10
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-10 (stromelysin 2) degrades a broad range of substrates including gelatin, collagen types III, IV and V, fibronectin, aggrecan, and pig cartilage proteoglycan. MMP-10 can activate other MMPs such as MMP-1 and MMP-8. MMP-10 is expressed in keratinocytes, T cells, menstrual endometrium, and a few tumor samples. Structurally, MMP-10 may be divided into four distinct domains: a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site; a short linker region, and a carboxyl terminal hemopexin-like domain.
Long Name
Matrix Metalloproteinase 10
Alternate Names
MMP10, Stromelysin 2
Entrez Gene IDs
4319 (Human)
Gene Symbol
MMP10
UniProt
Additional MMP-10 Products
Product Documents for Human MMP-10 Antibody
Product Specific Notices for Human MMP-10 Antibody
For research use only
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