Human MMP-14/MT1-MMP APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB9181A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 128527
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑14/MT1‑MMP
Tyr112-Ala541 (predicted)
Accession # P50281
Tyr112-Ala541 (predicted)
Accession # P50281
Specificity
Detects human MMP‑14/MT1‑MMP in direct ELISAs and Western blots. Does not detect E. coli-expressed recombinant human MMP-14 catalytic domain (aa 112-284).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human MMP-14/MT1-MMP APC-conjugated Antibody
Detection of MMP‑14/MT1‑MMP in MDA‑MB‑231 Human Cell Line by Flow Cytometry.
MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human MMP-14/MT1-MMP APC-conjugated Monoclonal Antibody (Catalog # FAB9181A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human MMP-14/MT1-MMP APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: MDA‑MB‑231 human breast cancer cell line
Sample: MDA‑MB‑231 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MMP-14/MT1-MMP
As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and integrin alphaV (1). MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplasmic tail (2).
References
- Seike, M. (2003) Cancer Lett. 194:1.
- Sato, H. et al. (1994) Nature 370:61.
Long Name
Matrix Metalloproteinase 14/Membrane Type 1 MMP
Alternate Names
MMP-X1, MMP14, MT-MMP1, MT1-MMP
Entrez Gene IDs
4323 (Human)
Gene Symbol
MMP14
UniProt
Additional MMP-14/MT1-MMP Products
Product Documents for Human MMP-14/MT1-MMP APC-conjugated Antibody
Product Specific Notices for Human MMP-14/MT1-MMP APC-conjugated Antibody
For research use only
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