Detects human MMP‑14/MT1‑MMP in direct ELISAs and Western blots. Does not detect E. coli-expressed recombinant human MMP-14 catalytic domain (aa 112-284).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human MMP-14/MT1-MMP PE-conjugated Antibody

Detection of MMP‑14/MT1‑MMP in MDA‑MB‑231 Human Cell Line by Flow Cytometry.

MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human MMP-14/MT1-MMP PE-conjugated Monoclonal Antibody (Catalog # FAB9181P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human MMP-14/MT1-MMP PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: MDA‑MB‑231 human breast cancer cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MMP-14/MT1-MMP

MMP-14/MT1-MMP (Matrix Metalloproteinase 14) is a member of the peptidase M10A family of enzymes. As such, it is one of some 25 zinc-dependent endopeptidases that are either soluble or membrane-bound. MMP-14 is expressed as a 63-64 kDa precursor that undergoes processing to generate either a 60 kDa catalytically-active transmembrane form, or a 55 kDa active soluble form. A number of substrates are reported for MMP-14, including Collagen I, II, and III, Laminin, Endoglin, Muc-1, Pro-MMP-13 and -2, and CD44. Molecules suggested to inhibit MMP-14 include TIMP-2, -3, and -4. Cells known to express MMP-14 include monocytes, platelets, osteoclasts, endothelial cells, fibroblasts, and muscle satellite cells. Over amino acids (aa) 112-284, human MMP-14 shares 98% aa sequence identity with mouse MMP-14.

Long Name

Matrix Metalloproteinase 14/Membrane Type 1 MMP

Alternate Names

MMP-X1, MMP14, MT-MMP1, MT1-MMP

Entrez Gene IDs

4323 (Human)

Gene Symbol

MMP14

UniProt

P50281

Additional MMP-14/MT1-MMP Products

Product Documents for Human MMP-14/MT1-MMP PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human MMP-14/MT1-MMP PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human MMP-14/MT1-MMP PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB9181P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications