Human MMP-17 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7796

R&D Systems, part of Bio-Techne
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MAB7796
MAB7796-SP
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Key Product Details

Species Reactivity

Human

Applications

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 823511

View all MMP-17 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP-17
Pro42-Ala534
Accession # Q9ULZ9

Specificity

Detects human MMP-17 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MMP-24 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human MMP-17 Antibody

Neutralization of Human MMP‑17 by Human MMP-17 Antibody.

Neutralization of Human MMP‑17 by Human MMP-17 Antibody.

The cleavage of Mca-PLAQAV-Dpa-RSSSR-NH2(10 µM, ES003) by Recombinant Human MMP-17 (1.0 ug/mL, 7796-MP) is measured after it has been preincubated with increasing concentrations of Mouse Anti-Human MMP-17 Monoclonal Antibody (Catalog # MAB7796). The ND50 is typically <9.0 µg/mL.

Applications for Human MMP-17 Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize Recombinant Human MMP-17 (1.0 μg/mL, Catalog # 7796-MP) cleavage of the fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 (10 μM, Catalog # ES003). The Neutralization Dose (ND50) is < 9.0 μg/mL.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-17

Matrix metalloproteinase 17 (MMP‑17) is a zinc metalloproteinase that is an integral membrane protein (1). It belongs to the membrane‑type MMP (MT‑MMP) subfamily, and is also known as MT4‑MMP. It is associated with the cell membrane by a glycosylphosphatidylinositol (GPI) anchor (1). It has the potential to function as a pro‑tumor necrosis factor‑ alpha (TNF‑ alpha) convertase (2). Unlike MMP‑14 (MT1‑MMP), it is a poor activator of pro‑MMP‑2. MMP‑17 is relatively poor at digesting components of the extracellular matrix but does cleave fibrinogen and fibrin (2). MMP‑17 is known to activate the aggrecanase ADAMTS‑4 through proteolysis (3). MMP‑17 is expressed in many tissues with the highest levels seen in the brain, colon, ovary, testis, and kidney papilla (4). It has been detected in several human cancers, including gliomas, prostate carcinomas, and breast carcinomas (5).

References

  1. Itoh, Y. et al. (1999) J. Biol. Chem. 274:34260.
  2. English, W. R. et al. (2000) J. Biol. Chem. 275:14046.
  3. Gao, G. et al. (2004) J. Biol. Chem. 279:10042.
  4. Srichai, M. B. et al. (2011) PLoS ONE 6:e17099.
  5. Sohail, A. et al. (2008) Cancer Metastasis Rev.  27:289.

Long Name

Matrix Metalloproteinase 17

Alternate Names

MMP17, MT4-MMP

Entrez Gene IDs

4326 (Human); 23948 (Mouse); 288626 (Rat)

Gene Symbol

MMP17

UniProt

Q9ULZ9 (Human)

Additional MMP-17 Products

Product Documents for Human MMP-17 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MMP-17 Antibody

For research use only

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