Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5869
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Xenograft
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Aldehyde Dehydrogenase 1-A1/ALDH1A1
Ser2-Ser501
Accession # P00352
Ser2-Ser501
Accession # P00352
Specificity
Detects human Aldehyde Dehydrogenase 1‑A1/ALDH1A1 in direct ELISAs and detects human and mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody
Detection of Human and Mouse Aldehyde Dehydrogenase 1‑A1/ALDH1A1 by Western Blot.
Western blot shows lysates of human liver tissue, human testis tissue, and mouse liver. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Aldehyde Dehydrogenase 1-A1/ALDH1A1 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Aldehyde Dehydrogenase 1-A1/ALDH1A1 in HeLa Human Cell Line.
Aldehyde Dehydrogenase 1-A1/ALDH1A1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Aldehyde Dehydrogenase 1‑A1/ALDH1A1 in Human Prostate Cancer Tissue.
Aldehyde Dehydrogenase 1-A1/ALDH1A1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Immunoprecipitation
25 µg/mL
Sample: Cell lysates spiked with Recombinant Human Aldehyde Dehydrogenase 1‑A1 (Catalog # 5869-DH), see our available Western blot detection antibodies
Sample: Cell lysates spiked with Recombinant Human Aldehyde Dehydrogenase 1‑A1 (Catalog # 5869-DH), see our available Western blot detection antibodies
Simple Western
10 µg/mL
Sample: Human liver tissue and mouse liver tissue
Sample: Human liver tissue and mouse liver tissue
Western Blot
1 µg/mL
Sample: Human liver tissue, human testis tissue, and mouse liver tissue
Sample: Human liver tissue, human testis tissue, and mouse liver tissue
Reviewed Applications
Read 1 review rated 5 using AF5869 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aldehyde Dehydrogenase 1-A1/ALDH1A1
Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that detoxify aldehydes by oxidizing them to carboxylic acids. Nineteen ALDHs are present in humans, expressed in a variety of organelles and having different substrate preferences (1). ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid (2). ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea (3). ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development (4). The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells (5). ALDH1A1 is a major enzyme in the oxidation of acetaldehyde, a toxic metabolite of ethanol (6).
References
Marchitti ,S.A. et al.- Zhao, D. et al. (1996) Eur. J. Biochem. 240:15.
- King, G. and R. Holmes (1997) Adv. Exp. Med. Biol. 414:19.
- Jacobs, F.M. et al. (2007) Development 134:2673.
- Chute, J.P. et al. (2006) Proc. Natl. Acad. Sci. USA 103:11707.
- Ueshima, Y. et al. (1993) Alcohol Alcohol. 1B:15.
Alternate Names
ALDC, Aldehyde Dehydrogenase 1A1, ALDH-E1, ALDH1A1, PUMB1, RALDH1
Entrez Gene IDs
216 (Human)
Gene Symbol
ALDH1A1
UniProt
Additional Aldehyde Dehydrogenase 1-A1/ALDH1A1 Products
- All Products for Aldehyde Dehydrogenase 1-A1/ALDH1A1
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 cDNA Clones
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 ELISA Kits
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 Lysates
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 Primary Antibodies
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 Proteins and Enzymes
Product Documents for Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody
Product Specific Notices for Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...