Human/Mouse BACE-1 Ectodomain Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9311

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
MAB9311
MAB9311-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunoprecipitation, Neutralization

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 137626

View all BACE-1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BACE-1
Thr22-Tyr460
Accession # P56817

Specificity

Detects human and mouse BACE‑1 Ectodomain in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) BACE-2, recombinant mouse BACE-2, rhADAM8, rhADAM9, rhADAM10, rhADAM15, or rhTACE is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human/Mouse BACE-1 Ectodomain Antibody

Neutralization of BACE‑1 Activity by Human/Mouse BACE‑1 Antibody.

Neutralization of BACE‑1 Activity by Human/Mouse BACE‑1 Antibody.

The cleavage of Mca-SEVNLDAEFRK(Dnp)RR-NH2(10 µM, Catalog # ES004) by Recombinant Human BACE-1 (10 µg/mL, Catalog # 931-AS) or Recombinant Mouse BACE-1 (10 µg/mL, Catalog # 2976-AS) is measured after preincubation with increasing concentrations of Mouse Anti-Human/Mouse BACE-1 Ectodomain Monoclonal Antibody (Catalog # MAB9311). The ND50 is typically 10 µg/mL.a

Applications for Human/Mouse BACE-1 Ectodomain Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human BACE‑1 (Catalog # 931‑AS) or Recombinant Mouse BACE‑1 (Catalog # 2976-AS), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Human BACE‑1 (10 µg/mL, Catalog # 931-AS) or Recombinant Mouse BACE‑1 (10 µg/mL, Catalog # 2976-AS) cleavage of the fluorogenic peptide substrate Mca-SEVNLDAEFRK(Dnp)RR-NH2 (10 µM, Catalog # ES004). The Neutralization Dose (ND50) is typically 10 µg/mL.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BACE-1

BACE-1 (beta‑site APP cleaving enzyme-1) is an aspartic protease and an integral membrane protein (1, 2). It is the major beta secretase, and together with the gamma secretase, is responsible for generating the amyloid beta peptide (A beta) from the amyloid precursor protein (APP) (3, 4). Because A beta is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. High levels of BACE-1 activity are sufficient to elicit neurodegeneration and neurological decline in vivo, indicating that inhibiting BACE-1 may block not only A beta-dependent but also A beta-independent pathogenic mechanisms (5). In addition to APP, BACE-1 also cleaves APP-like proteins 1 and 2, the cell adhesion protein P-selectin glycoprotein ligand-1 and beta-galactoside alpha2,6-sialyltransferase, implying that BACE-1 may have additional functions involving the ectodomain shedding of membrane proteins (6 - 8).

References

  1. Vassar, R. et al. (1999) Science 286:735.
  2. Yan, R. et al. (1999) Nature 402:533.
  3. Cai, H. et al. (2001) Nature Neurosci. 4:233.
  4. Roberds, S.L. et al. (2001) Human Mol. Genet. 97:1317.
  5. Rockenstein, E. et al. (2005) J. Biol. Chem. 280:32957.
  6. Li, Q and T.C. Sudhof (2004) J. Biol. Chem. 279:10542.
  7. Lichtenthaler, S.F. et al. (2003) J. Biol. Chem. 278:48713.
  8. Kitazynem, S. et al. (2005) J. Biol. Chem. 280:8589.

Long Name

beta-site Ayloid Precursor Protein Cleaving Enzyme 1

Alternate Names

BACE1

Entrez Gene IDs

23621 (Human); 23821 (Mouse)

Gene Symbol

BACE1

UniProt

P56817

Additional BACE-1 Products

Product Documents for Human/Mouse BACE-1 Ectodomain Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse BACE-1 Ectodomain Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.