Human/Mouse Bcl-w Antibody
R&D Systems, part of Bio-Techne | Catalog # AF824
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
KLH-coupled human/mouse Bcl-w synthetic peptide
Gly32-Asp51
Accession # Q92843
Gly32-Asp51
Accession # Q92843
Specificity
Detects human and mouse Bcl-w in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse Bcl-w Antibody
Detection of Human and Mouse Bcl‑w by Western Blot.
Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1.2 µg/mL Rabbit Anti-Human/Mouse Bcl-w Antigen Affinity-purified Polyclonal Antibody (Catalog # AF824) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Bcl-w Minus C-Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.Applications for Human/Mouse Bcl-w Antibody
Application
Recommended Usage
Western Blot
1.2 µg/mL
Sample: WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line
Sample: WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bcl-w
Bcl-w, also called Bcl-2 like protein 2, is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
References
- Gross, A. et al. (1999) Genes and Develop. 13:1899.
- Kroemer, G. (1997) Nature Med. 3:614.
- Ross, J.A., et al. (1998) Nat. Genet. 18:251.
-
Print, C.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12323.
-
Yan, W. et al. (2000) Mol. Endocrin. 14:682.
Long Name
B Cell Lymphoma/Leukemia w
Alternate Names
BCL2L2, Bclw
Gene Symbol
BCL2L2
UniProt
Additional Bcl-w Products
Product Documents for Human/Mouse Bcl-w Antibody
Product Specific Notices for Human/Mouse Bcl-w Antibody
For research use only
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