Human/Mouse BMP-10 Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # MAB2926
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Neutralization, Western Blot
Cited:
ELISA (detection), ELISA Capture, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 462732
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human BMP-10
Asn317-Arg424
Accession # O95393
Asn317-Arg424
Accession # O95393
Specificity
Detects human BMP-10 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BMP-1.1, 2, 3, 3b, 4, 5, 6, 7, 9, 15, or the pro region of rhBMP-10 is observed. In direct ELISAs, approximately 90% cross-reactivity with recombinant mouse BMP-10 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human/Mouse BMP-10 Antibody
Alkaline Phosphatase Production Induced by BMP-10 and Neutralization by Human BMP-10 Antibody.
Recombinant Human BMP-10 (Catalog # 2926-BP) induces alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-10 (100 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP-10 Monoclonal Antibody (Catalog # MAB2926). The ND50 is typically 0.15-0.9 µg/mL.Applications for Human/Mouse BMP-10 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human BMP-10 (Catalog # 2926-BP)
under non-reducing conditions only
Sample: Recombinant Human BMP-10 (Catalog # 2926-BP)
under non-reducing conditions only
Neutralization
Measured by its ability to neutralize BMP‑10-induced alkaline phosphatase production in the MC3T3‑E1 mouse preosteoblast cell line. The Neutralization Dose (ND50) is typically 0.15-0.9 µg/mL in the presence of 100 ng/mL Recombinant Human BMP‑10.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-10
Long Name
Bone Morphogenetic Protein 10
Alternate Names
BMP10
Gene Symbol
BMP10
UniProt
Additional BMP-10 Products
Product Documents for Human/Mouse BMP-10 Antibody
Product Specific Notices for Human/Mouse BMP-10 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...