Human/Mouse c-Myc Antibody

c‑Myc in D3 Mouse Stem Cells.

c-Myc was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/Mouse c-Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

c-Myc in BG01V Human Stem Cells.

c-Myc was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human/Mouse c-Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human c-Myc by Simple Western^TM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for c-Myc at approximately 78 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse c-Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human c‑Myc Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and c-Myc knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse c-Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for c-Myc at approximately 52 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse c-Myc by Western Blot

DON significantly inhibits xenograft tumor growth.(A) SK-N-AS tumors were treated with DON at 100 mg/kg or water by i.p. twice weekly. Weight loss in DON-treated mice reduced the treatment cohort to 2 mice indicated by (2) at later timepoints. (B) SK-N-FI tumors were treated with DON at 100 mg/kg, 50 mg/kg or water by i.p. twice weekly or 100mg/kg once a week (100mg/kg; 1x/wk). The * indicates p