Human/Mouse DAPP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7024
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 716804
Product Summary for Human/Mouse DAPP1 Antibody
Immunogen
E. coli-derived recombinant human DAPP1
Gly2-Ser163
Accession # Q9UN19
Gly2-Ser163
Accession # Q9UN19
Specificity
Detects human DAPP1 in direct ELISAs. Detects human and mouse DAPP1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse DAPP1 Antibody
Detection of Human and Mouse DAPP1 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse DAPP1 Monoclonal Antibody (Catalog # MAB7024) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DAPP1 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse DAPP1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A431 human epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and BaF3 mouse pro-B cell line
Sample: A431 human epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and BaF3 mouse pro-B cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DAPP1
Long Name
Dual Adaptor of Phosphotyrosine and 3-Phosphoinositides 1
Alternate Names
BAM32
Gene Symbol
DAPP1
UniProt
Additional DAPP1 Products
Product Documents for Human/Mouse DAPP1 Antibody
Product Specific Notices for Human/Mouse DAPP1 Antibody
For research use only
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