Human/Mouse DGK-iota Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6435
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 648538
Product Specifications
Immunogen
E. coli-derived recombinant human DGK‑ iota
Ser925-Val1065 (predicted)
Accession # O75912
Ser925-Val1065 (predicted)
Accession # O75912
Specificity
Detects human and mouse DGK‑ iota in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human DGK-alpha, -beta, -delta, -epsilon, -eta, -gamma, -kappa, -theta, or - zeta is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse DGK-iota Antibody
Detection of Human and Mouse DGK‑ iota by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and mouse brain tissue. PVDF Membrane was probed with 1 µg/mL of Human/Mouse/Rat DGK-iota Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB6435) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-iota at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse DGK-iota Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line and mouse brain tissue
Sample: SH‑SY5Y human neuroblastoma cell line and mouse brain tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DGK-iota
Long Name
Diacylglycerol Kinase, iota 64kDa
Alternate Names
DGKI, DGKiota
Gene Symbol
DGKI
UniProt
Additional DGK-iota Products
Product Documents for Human/Mouse DGK-iota Antibody
Product Specific Notices for Human/Mouse DGK-iota Antibody
For research use only
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