Human/Mouse ELF3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5787
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 662533
Product Specifications
Immunogen
E. coli-derived recombinant human ELF3
Met1-Gly173
Accession # P78545
Met1-Gly173
Accession # P78545
Specificity
Detects human and mouse ELF3 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse ELF3 Antibody
Detection of Human and Mouse ELF3 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, EL-4 mouse lymphoblast cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ELF3 Monoclonal Antibody (Catalog # MAB5787) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ELF3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse ELF3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, EL‑4 mouse lymphoblast cell line, and A549 human lung carcinoma cell line
Sample: Jurkat human acute T cell leukemia cell line, PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, EL‑4 mouse lymphoblast cell line, and A549 human lung carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ELF3
ELF3 (E74-Like Factor 3), also known as ESE-1, ESX, ERT and JEN, is a 41-43 kDa member of the ETS family of proteins. During uncomplicated (non-inflammatory) periods of cell differentiation, ELF3 is expressed exclusively by epithelial cells, repressing genes needed during early differentiation, and promoting genes needed for full differentiation. Under conditions of inflammation, cells such as monocytes, endothelial cells and chrondrocytes express ELF3 and produce molecules such as Ang1 and COX2. Human ELF3 is 371 amino acids (aa) in length. It contains one PNT/pointed dimerization domain (aa 46-132), a protein stabilizing PEST sequence (aa 210-225), an A/T Hook region that binds to AT-rich DNA sequences (aa 236-252), and an ETS DNA binding domain (aa 273-355). ELF3 interacts with CREBBP, EP300, KU70 and KU86. There is one splice variant that shows a deletion of aa 174-200. Over aa 1-173, human ELF3 shares 87% aa identity with mouse ELF3.
Long Name
E74-like factor 3
Alternate Names
EPR1, ERT, ESX, JEN
Gene Symbol
ELF3
UniProt
Additional ELF3 Products
Product Documents for Human/Mouse ELF3 Antibody
Product Specific Notices for Human/Mouse ELF3 Antibody
For research use only
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