Human/Mouse FABP4/A-FABP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3150
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
ELISA, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 804914
Product Specifications
Immunogen
E. coli-derived recombinant human FABP4/A‑FABP
Met1-Ala132
Accession # P15090
Met1-Ala132
Accession # P15090
Specificity
Detects human FABP4/A‑FABP in direct ELISAs and human and mouse FABP4/A-FABP in Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) FABP4, 50% cross-reactivity with recombinant human (rh) FABP3, 15% cross-reactivity with rhFABP3, and no cross-reactivity with rhFABP8 or rhFABP9 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Human/Mouse FABP4/A-FABP Antibody
Detection of Human and Mouse FABP4/A‑FABP by Western Blot.
Western blot shows lysates of human adipose tissue and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human FABP4/A-FABP Monoclonal Antibody (Catalog # MAB3150) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Human/Mouse FABP4/A-FABP ELISA Standard Curve.
Recombinant Human/Mouse FABP4/A-FABP protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse FABP4/A-FABP Monoclonal Antibody (Catalog # MAB3150) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (AF3150) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).Applications for Human/Mouse FABP4/A-FABP Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human FABP4/A-FABP DuoSet ELISA Kit (Catalog # DY3150-05) for convenient development of a sandwich ELISA or the Human FABP4 Quantikine ELISA Kit (Catalog # DFBP40) for a complete optimized ELISA.
Western Blot
1 µg/mL
Sample: Human adipose tissue and mouse adipose tissue
Sample: Human adipose tissue and mouse adipose tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP4/A-FABP
Long Name
Fatty Acid-Binding Protein 4
Alternate Names
A-FABP, AFABP, aP2
Gene Symbol
FABP4
UniProt
Additional FABP4/A-FABP Products
Product Documents for Human/Mouse FABP4/A-FABP Antibody
Product Specific Notices for Human/Mouse FABP4/A-FABP Antibody
For research use only
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